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KMID : 0617819930030010065
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Journal of Wonkwang Oriental Medicine
1993 Volume.3 No. 1 p.65 ~ p.80
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Study on the Cytotoxic Effect of Pinelliae Tuber Extracts according to the Prebrewed Methods
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李秀培/Lee, Soo-Bae
金德宗/金康柱/田炳勳/禹元洪/Kim, Deog-Jong/Kim, Kang-Ju/Jeon, Byoung-Hun/Woo, Won-Hong
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Abstract
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The purpose of this study is to know the effects of Pinelliae Tuber on the cytotoxicity of human gingival fibroblasts according to brewing methods. Pinelliae Tuber was extracted by water in the state of being raw materials, prebrewing with Zingiberis Tuber and alumen. The extracted Pinelliae Tuber was applied to the cultured human gingival fibroblasts.
The cytotoxic effct of Pinelliae Tuber was meaeured by MTTassay and microscopic findings.
The results were as follows :
1. The cell viability of the fibroblasts by Pinelliae Tuber water extracts significantly decreased compared to control group.
2. The cell viability by the Pinelliae Tuber extracts prebrewed with Zingiberis Tuber was similiar to those of control group.
3. The cell viability by the Pinelliae Tuber extracts prebrewed with alumen slightly decreased, but was higher than those of PT group.
4. According to the microscopec findings, the number of cell count were smaller than normal in all experimental group, specially in the PT group, and showed the shrinkage of cytoplasmic process in the PT group and PA group.
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